Managing the Care of Patients With Diabetes in the Home Care Setting
Caryl Ann O'Reilly
Jul 1, 2005; 18:162-166
Articles
A neuroglobin-based high-affinity ligand trap reverses carbon monoxide-induced mitochondrial poisoning [Molecular Biophysics]
Carbon monoxide (CO) remains the most common cause of human poisoning. The consequences of CO poisoning include cardiac dysfunction, brain injury, and death. CO causes toxicity by binding to hemoglobin and by inhibiting mitochondrial cytochrome c oxidase (CcO), thereby decreasing oxygen delivery and inhibiting oxidative phosphorylation. We have recently developed a CO antidote based on human neuroglobin (Ngb-H64Q-CCC). This molecule enhances clearance of CO from red blood cells in vitro and in vivo. Herein, we tested whether Ngb-H64Q-CCC can also scavenge CO from CcO and attenuate CO-induced inhibition of mitochondrial respiration. Heart tissue from mice exposed to 3% CO exhibited a 42 ± 19% reduction in tissue respiration rate and a 33 ± 38% reduction in CcO activity compared with unexposed mice. Intravenous infusion of Ngb-H64Q-CCC restored respiration rates to that of control mice correlating with higher electron transport chain CcO activity in Ngb-H64Q-CCC–treated compared with PBS-treated, CO-poisoned mice. Further, using a Clark-type oxygen electrode, we measured isolated rat liver mitochondrial respiration in the presence and absence of saturating solutions of CO (160 μm) and nitric oxide (100 μm). Both CO and NO inhibited respiration, and treatment with Ngb-H64Q-CCC (100 and 50 μm, respectively) significantly reversed this inhibition. These results suggest that Ngb-H64Q-CCC mitigates CO toxicity by scavenging CO from carboxyhemoglobin, improving systemic oxygen delivery and reversing the inhibitory effects of CO on mitochondria. We conclude that Ngb-H64Q-CCC or other CO scavengers demonstrate potential as antidotes that reverse the clinical and molecular effects of CO poisoning.
A neuroglobin-based high-affinity ligand trap reverses carbon monoxide-induced mitochondrial poisoning [Molecular Biophysics]
Carbon monoxide (CO) remains the most common cause of human poisoning. The consequences of CO poisoning include cardiac dysfunction, brain injury, and death. CO causes toxicity by binding to hemoglobin and by inhibiting mitochondrial cytochrome c oxidase (CcO), thereby decreasing oxygen delivery and inhibiting oxidative phosphorylation. We have recently developed a CO antidote based on human neuroglobin (Ngb-H64Q-CCC). This molecule enhances clearance of CO from red blood cells in vitro and in vivo. Herein, we tested whether Ngb-H64Q-CCC can also scavenge CO from CcO and attenuate CO-induced inhibition of mitochondrial respiration. Heart tissue from mice exposed to 3% CO exhibited a 42 ± 19% reduction in tissue respiration rate and a 33 ± 38% reduction in CcO activity compared with unexposed mice. Intravenous infusion of Ngb-H64Q-CCC restored respiration rates to that of control mice correlating with higher electron transport chain CcO activity in Ngb-H64Q-CCC–treated compared with PBS-treated, CO-poisoned mice. Further, using a Clark-type oxygen electrode, we measured isolated rat liver mitochondrial respiration in the presence and absence of saturating solutions of CO (160 μm) and nitric oxide (100 μm). Both CO and NO inhibited respiration, and treatment with Ngb-H64Q-CCC (100 and 50 μm, respectively) significantly reversed this inhibition. These results suggest that Ngb-H64Q-CCC mitigates CO toxicity by scavenging CO from carboxyhemoglobin, improving systemic oxygen delivery and reversing the inhibitory effects of CO on mitochondria. We conclude that Ngb-H64Q-CCC or other CO scavengers demonstrate potential as antidotes that reverse the clinical and molecular effects of CO poisoning.
She’s running? Cardi B: ‘I think I want to be a politician.’
Cardi B pondered political life in a series of tweets Sunday.
Cardi B and Bernie Sanders talk police brutality and health care — while in a nail salon
The senator from Vermont and the rapper bantered and got into the issues in a new campaign video.
Cardi B continues her support of Bernie Sanders by filming a campaign video together
The rapper has been a supporter of the Democratic presidential candidate, and says she wants to help "a movement of young people to transform this country."
Cartoonists share their secrets to drawing the Democratic candidates, from Bernie’s hair to Klobuchar’s smile
“People who have the most exaggerated features tend to make the worst caricature subjects, and in that sense, I hate drawing Bernie,” says one.
Cartoonists are having a tougher time with Trump’s impeachment than with Clinton’s. Except when it’s easier.
Cartoonists who have lampooned both administrations take aim differently now.
Car caught going 124 km/h over speed limit on Hwy. 174
Ottawa Police hit the brakes on a motorist going 124 km/h over the posted speed limit in Ottawa’s east-end.
IndyStar Sports Awards transforms to on-demand broadcast, loaded with star pro athletes
Carmel and IU grad Sage Steele will co-host and star athletes like Drew Brees and Venus Williams will announce winners during the online broadcast.
IU women's basketball lands high-scoring grad transfer Nicole Cardaño-Hillary
Cardaño-Hillary leaves George Mason as the school's all-time leading scorer and was named Atlantic 10 Player of the Year last season.
Caracas, Bogota elçisini çekiyor
Venezuela lideri Hugo Chavez, sınır komşusu Kolombiya'daki büyükelçisini geri çekeceğini ve iki ülke arasındaki ilişkilerin dondurulacağını açıkladı. Kolombiya, Caracas'ı FARC'a silah temin etmekle suçlamıştı.
IndyStar Sports Awards transforms to on-demand broadcast, loaded with star pro athletes
Carmel and IU grad Sage Steele will co-host and star athletes like Drew Brees and Venus Williams will announce winners during the online broadcast.
Carregador de caixão dançarino de Gana celebra memes, mas lamenta pandemia: 'Derrubou meu negócio'
Repórter da BBC volta a entrevistar líder de grupo após vídeo que virou meme.
Cartoonist Gary Varvel: Democratic Scrooge
Funding for Trump's border wall is met with resistance
Cartoonist Gary Varvel: Mayor Hogsett's 12 days of Christmas
A reelection campaign song
Cartoonist Gary Varvel: What Democrats want for Christmas
Will the Mueller investigation deliver?
Cartoonist Gary Varvel: Trump is Tariff Man
The president claims to have super trade powers
Cartoonist Gary Varvel: George Bush 41
Reunited with Barbara and their daughter Robin
Cartoonist Gary Varvel: GM workers losing jobs
General Motors announces 14,000 job cuts
Cartoonist Gary Varvel: The count in Florida
The Sunshine State could use some help counting votes.
Cartoonist Gary Varvel: Political script
The minority party may change but the rhetoric stays the same
Cartoonist Gary Varvel: California firefighters
A great crisis produces great people and great courage
Cartoonist Gary Varvel: A blue wave in the U.S. House
Could a Trump investigation wash up?
Cartoonist Gary Varvel: Thousand Oaks bar shooting
America mourns another mass shooting.
Cartoonist Gary Varvel: A history of voter cartoons
The importance of voting as seen in Varvel's cartoons.
Cartoonist Gary Varvel: Voter alert
An important reminder to exercise your civic duty
Cartoonist Gary Varvel: Negative campaign commercials
Many voters are turned off by political attacks.
Cartoonist Gary Varvel: Early voters
A caravan heads to the polls. Voter turnout is already at record levels nationally.
Cartoonist Gary Varvel: What's powering Indiana's Senate race
Braun and Donnelly tout their support for Trump's policies
Cartoonist Gary Varvel: Politics of a foreign kind puzzle voters
Donnelly and Braun campaigns focus on personal attacks
Cartoonist Gary Varvel: Trump's magic wand
Obama downplays Trump's economic successes
Carmel partners with company to test residents' feces for coronavirus
How many people have coronavirus in Carmel? The city soon may soon get a better picture — by testing residents' feces.
Carmel mayor's promotion of coronavirus testing lab Aria Diagnostics raises concerns
Some ethics experts say Carmel Mayor Jim Brainard's push for coronavirus testing at Indianapolis lab Aria Diagnostics raises flags.
Here's what Carmel, Indiana, looks like during the coronavirus pandemic
Carmel, Indiana, copes with closures during the COVID-19 pandemic on Wednesday, April 22, 2020.
Carmel gears up for summer farmers market during coronavirus pandemic
Ron Carter, president of Carmel Farmers Markets, talks about the city's preparations for the summer market amid worries over the novel coronavirus.
Carmel Farmers Market stays open, urges shoppers and vendors to be 'as safe as possible'
The Carmel Farmers Market will be open this weekend, but with safety precautions in place.
Letters: Taxpayers foot the bill for 'fancy' roundabouts in Carmel
Carmel is spending too much taxpayer money building expensive roundabouts when simpler ones would be enough, a letter to the editor says.
Carl Thayer: 'Vết thương vẫn chưa lành sau 45 năm Cuộc chiến VN'
Vấn đề hoà giải, hoà hợp vẫn còn nhức nhối giữa những người Việt thuộc hai phe, GS Carl Thayer, nhà nghiên cứu chính trị VN và bang giao quốc tế, nói.
